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Abstract

Chitosan was synthesized from Apis Mellifera dead bees by traditional and cryogenic methods, and the degree of deacetylation, viscosity, and molecular mass of the synthesized sample was studied. Chitosan was synthesized by deacetylation of chitin under cryogenic conditions. NaOH concentration 40%, temperature 90 ± 3 °C, time 120 min. The degree of deacetylation, and molecular mass was determined using an Ubbelode viscometer. DDA was determined by conductometric titration under standard conditions. The degree of deacetylation of Apis Mellifera chitosan was 87%. synthesized by cryogenic method DDA of chitosan was 83% synthesized by traditional method. Conducting the synthesis in liquid nitrogen atmosphere leads to a certain decrease in the molecular mass of chitosan. This research aims to study the important parameters of chitosan molecule such as deacetylation level, viscosity and molecular mass.

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